Method of quantitation rapid micro - organisms coliformes living in a sample of water.

摘要

The present invention relates to a method of quantitation rapid micro - organisms coliformes living in a sample of water in which one places the micro - organisms in contact with a fluorogenic substrate which is hydrolyzed in methylumbelliferone by an enzyme produced by the microphone - organisms. The methylumbelliferone is detected by the fluorescence that it emits, the speed of variation of the fluorescence being measured in order to determine the concentration of the micro - organisms coliformes in the sample of origin from the speed of variation calculated and a diagram of corrélation.de characteristic manner, a preliminary step of treatment, especially a pre - filtration, the sample which makes it possible to remove at least a portion of the particles in suspension in water, which makes it possible to give a very similar to that of the standardized method by microplaque.application to the analysis of the water of swimming for the detection of escherichia coli.