METHOD FOR PREPARING Spo11 RELATED PROTEIN OF HIGHER PLANT WITH DNA-BINDING FUNCTION

摘要

PROBLEM TO BE SOLVED: To provide a soluble Spo11 protein holding a DNA-binding function and a method for preparing the same.;SOLUTION: This method for preparing the soluble Spo11 protein holding the DNA-binding function is provided by expressing the Spo11 protein as a form of being fused with a trigger factor (TF) originated from E. coli, recovering the fused protein as a soluble fraction of the E. coli extract, and further through a purification process including a process of a heparin column chromatography, preparing the soluble Spoll protein holding the DNA-binding property. The Spoll protein prepared by the above method is also provided.;COPYRIGHT: (C)2008,JPO&INPIT