3 'modified oligonucleotides containing pseudoisocytosine isocytosine nucleic acid base derivative, and its application as a probe or primer

摘要

In particular, a primer containing a modified nucleic acid base specific 3 'end of the primer region to reduce the formation of primer dimers in the amplification reaction, and I disclose that a variety of usage. In one embodiment, the 4 nucleotides within the 3 'end, a polynucleotide according to the invention comprises a pyrimidine nucleobase at least one modified. In another embodiment, the present invention provides a method for detecting primer extension, oligonucleotide ligation method, the target polynucleotide sequence using a modified nucleic acid bases thereof.