ATP method for inspecting and weighing a cell quantity which is based on nukureochidoadeniriru inside the cell, utilization and method for deciding the especially non ATP germ

摘要

SolutionsThis invention can exist in the sample liquid of kind of given, lives reaction formula for inspecting and weighing a cell quantity which (1) the luciferin +ATP+O2 +Mg2++ the luciferase - with the oxy luciferin + photon regarding the utilization of the bioluminescence being something, this utilization is not, non virus kind of given you live is displayed in the form of ATP of the cell where free nukureochidoadeniriru inside the cell which (AN) measurement of all content, the addition (i) ATP with after the adding ATP of the (ii) known quantity to be done, the total of free ATP, ADP and AMP inside the cell of the aforementioned family, myokinase and the pill buildingAfter freedom ADP inside the cell and AMP convert to ATP with the acid kinase, the fact that the point that is utilized it is fixed relational expression (2) [AN] = [ATP] + [ADP] + [AMP] with =Cte is feature. In addition, it regards the method of inspecting and weighing a cell quantity with ATP method.