Ribosome recycling factor of plant

摘要

PROBLEM TO BE SOLVED: To obtain a new frr gene which consists of a specific gene coding for spinach chloroplast ribosome recycling factor(RRF), and is useful, for example, for the development of next-generation RRF-targeting antibiotics and herbicides. ;SOLUTION: This is a new frr gene which codes for spinach chloroplast ribosome recycling factor(RRF) having a base sequence shown by the formula, and is useful, for example, for the development of next-generation RRF-targeting antibiotics and herbicides. The frr gene is obtained by screening a spinach cDNA library using an antibody against the chloroplast envelope membrane, purifying the obtained positive clones, and cloning by PCR using primers to both ends of the cloning site.;COPYRIGHT: (C)1999,JPO