Expression of cytochrome P450 in the intestinal bacteria

摘要

A bacterial cell comprising (57) a P450 monooxygenase system [Abstract] functional cytochrome, the genetic construct which is capable of expressing a cytochrome P450 reductase separately from the cytochrome P450 gene construct in which the cells are capable of expressing cytochrome P450 includes things, has been adapted to to allow functional coupling of said cytochrome P450 and said cytochrome P450 reductase in the cells within each of the N-terminus of the cytochrome P450 reductase and the N-terminus of the cytochrome P450. In bacterial cells, including cytochrome P450, it contains a genetic construct capable of expressing and encodes the cytochrome P450, cytochrome P450 can comprise an N-terminal portion which directs the cytochrome P450 to a cellular compartment or membrane of the bacterial cell. For example, as a bioreactor, in carcinogenicity tests and drug testing, and as a source of cytochrome P450, the fungus cells are useful.