Methods for labeling the sequence-specific nucleic acids, and new nucleic acid detection method using the same

摘要

It is possible to provide [challenge] the technique for detecting nucleic acid in a simple manner. A method for producing a nucleic acid has been introduced [SOLUTION] label, the following steps: comprising the steps of providing a template nucleic acid and A) nucleic acid to be labeled, the template type nucleic acids of the nucleic acid to be labeled It is complementary to at least a portion, having a nucleotide long one nucleotide at least in part to the tip of at least one step of the nucleic acid to be labeled; more to be labeled allowed to hybridize with the template type nucleic acid, a B) the nucleic acid to be labeled process to generate a template nucleic acid complex - nucleic acid; C) the nucleic acid to be labeled - the method comprising the template nucleic acid complex, to an extension reaction, the addition of labeled nucleotides and nucleic acid synthesis enzyme, at least in the nucleic acid to be labeled nucleotides are the labeled one is taken based on the template type nucleic acid, process; comprising the step, of extracting the nucleic acid to be labeled extended from the mixture after D) long reaction was generated, methods. [Selection] Figure Figure 1