Production manner null of the activated blood clotting VIIth factor which is based on the activated manner and the said manner of blood clotting VIIth

摘要

PROBLEM TO BE SOLVED: To extremely easily produce an activated blood coagulation factor VII having depressed contaminant protein content by treating a plasma fraction containing blood coagulation factor VII by anion exchange chromatography. SOLUTION: A solution containing a blood coagulation factor VII (FVII) is brought into contact with an anion exchange resin and developed. The developed FVII is eluted by partially activating the FVII. The activation rate is preferably 60%. The eluate is left at rest in a solution state to completely activate the non-activated molecule. The buffer solution used in the elution preferably contains Ca2+ at a concentration of =0.5mM. The leaving (aging) of the eluate solution is preferably carried out in a solution containing =0.5mM of Ca2+ until. =95% of the FVII is activated. The formation of contaminant substance in the activation process can be suppressed to enable remarkable increase in the yield of the activated product.