Parthenolide attenuates LPS-induced activation of NF-κB in a timedependent manner in rat myocardium

摘要

Parthenolide(PTN),a selective nuclear factor kappa B(NF-κB)inhibitor,has been used extensively to inhibit NF-κB activation.The duration of the inhibitory effect of PTN on NF-κB in vivo remains unclear.This study was to determine whether a lipopolysaccharide(LPS)challenge 6,12 and 24 h after the administration of PTN could activate NF-κB.Rats were devided into five groups.The rats in the PTN,PTN+LPS and DMSO groups were injected intraperitoneally with PTN or DMSO.After 6,12 or 24 h,LPS was administered in LPS and PTN+LPS groups.The expressions of NF-κB p50,IκBα and p-IκBα were inhibited in both PTN and PTN+LPS group at end of 6 and 12 h and no effects at 24 h.In summary,myocardial NF-κB expression occurs 1 h after the administration of LPS.PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo.