Cell release method, cell release solution, cell culture method, cell culture solution, cell solution, cell solution preparation, cell fixing method and cell fixing solution

摘要

An object of the present invention is to provide a method in which adherent animal cells are normally cultured and proliferated in a floating state, and the cultured and proliferated adherent animal cells are re-established in a desired place. A cell release method according to the present invention is characterized in that lactoferrin is added to a culture solution of a culture vessel in which cells are cultured. In addition, the cell culture method according to the present invention is characterized in that cells are suspended and cultured in a culture solution containing lactoferrin. The cell fixing method according to the present invention is characterized by diluting lactoferrin, transferring a culture solution containing floating cells to a laminin-coated incubator, or adding glycosaminoglycan. In these, the cells are adherent animal cells, and the culture medium contains serum. [Selection] Figure 1