Quantification method by immobilized enzyme chip using His-Tag fusion phenylalanine dehydrogenase L- phenylalanine

摘要

Phenylalanine dehydrogenase fused to the N-terminal oligopeptide consisting of histidine of 3 to 12 pieces. Phenylalanine dehydrogenase was fused to the C-terminus of the oligopeptide consisting of histidine of 3 to 12 pieces. Having a plurality of wells on the substrate surface, and in these wells, the immobilized enzyme chip with the immobilized phenylalanine dehydrogenase. And a plurality of wells on the substrate surface, the wells of these immobilized phenylalanine dehydrogenase fused to the N-terminus an oligopeptide consisting of histidine 6-9 pieces L- phenylalanine analysis immobilized enzyme chip. It comprises in the wells of the immobilized enzyme chip, resazurin, diaphorase, and the Inkyube~yon with the reaction solution containing (NAD) nicotinamide adenine dinucleotide test sample, detects the color of the reaction solution, the test sample analytical methods L- phenylalanine contained.