Specific detection method for bacteria belonging to the genus Alicyclobacillus

摘要

An object of the present invention is to provide a rapid, simple, and low-cost method for specifically detecting Alicyclobacillus bacteria in distinction from other bacteria. (1) For a test sample, a primer set consisting of an oligonucleotide having the base sequence shown in SEQ ID NO: 1 and an oligonucleotide having the base sequence shown in SEQ ID NO: 2, or an oligonucleotide having the base sequence shown in SEQ ID NO: 3 and SEQ ID NO: A step of performing PCR using a primer set comprising an oligonucleotide having the base sequence shown in 4, (2) hybridizing the obtained PCR product with a probe comprising an oligonucleotide having the base sequence shown in SEQ ID NO: 5 or 6 And (3) a method for specifically detecting a bacterium belonging to the genus Alicyclobacillus, comprising a step of detecting the hybridized product obtained above.