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Method Of Measuring the Number Of Bacteria, Device Of Measuring the Number Of Bacteria and Cell Used In the Device

机译:测定细菌数的方法,测定细菌数的装置以及装置中使用的细胞

摘要

A method of measuring the number of bacteria according to the present invention includes step (a) through step (d). First, in step (a), a to-be-measured sample (specimen) including a predetermined bacterial strain (such as Escherichia coli or coliform bacteria) is added to a predetermined medium (such as a medium used for a specific enzyme substrate culture medium method). In step (b), a current value flowing through the medium added with the sample is measured with an oxygen electrode at a predetermined temperature and at a predetermined constant voltage. In step (c), time required is measured for the current value that has decreased temporarily after starting the measurement of step (b) to increase thereafter to exceed a predetermined threshold value. In step (d), the initial number of bacteria of the bacterial strain included in the sample is calculated based on the time required.
机译:根据本发明的测量细菌数目的方法包括步骤(a)至步骤(d)。首先,在步骤(a)中,将包括预定细菌菌株(例如大肠埃希氏菌或大肠菌)的待测样品(样品)添加到预定培养基(例如培养基)中。用于特定酶底物培养基的方法)。在步骤(b)中,用氧电极在预定温度和预定恒定电压下测量流过添加有样品的介质的电流值。在步骤(c)中,测量在开始步骤(b)的测量之后暂时减小的电流值随后增加以超过预定阈值所需的时间。在步骤(d)中,基于所需时间计算样品中包括的细菌菌株的初始细菌数。

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