Production of a Polypeptide in a Serum-Free Cell Culture Liquid Containing Plant Protein Hydrolysate

摘要

The invention concerns a method for large-scale production of a polypeptide in eukaryote cells contained in a serum-free culture liquid, said method comprising (i) a propagation phase for said cells, where the cells are propagated in a first cell culture liquid, and (ii) a production phase for said cells, where the cells are present in a second cell culture medium liquid, wherein each of the cell culture liquids comprise a plant protein hydrolysate, and wherein the ratio between the concentration of the plant protein hydrolysate (C₁) in the first cell culture liquid and the concentration of the plant protein hydrolysate (C₂) in the second cell culture liquid is at least 1.5:1 (C₁:C₂).