Propagation in Serum-Free Medium of Cell Cultures Useful for Vaccine Production.

摘要

Serum-free media were developed for the growth of diploid fetal Rhesus monkey lung (DBS-FRhL-2) cells and for the propagation of dengue type 2 virus, vaccine strain (dengue-2 vaccine virus). The serum originally required in the medium was substituted with a combination of vitamins, amino acids, growth factors, hormones, other organic compounds, and inorganic salts. Three other cells were adapted to grow serially in serum-free medium: (1) rhesus monkey kidney cells (MK-2, LLC-1) using our WO5 medium in shaker and monolayer cultures; (2) human lung; and (3) human foreskin cells (diploid) in WO20 medium in monolayers. There was not sufficient time to test these cells for their susceptibility and determination of peak titer of dengue-2 parent and vaccine viruses.