NOVEL METASTASIS SUPPRESSOR GENE ON HUMAN CHROMOSOME 8

摘要

An isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence encoding the metastasis suppressor gene located at p21-p12 on chromosome 8 of a human (Tey 1), a variant Tey 1, or a fragment of either of the foregoing comprising at least 455 contiguous nucleotides; an isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence that is complementary to a nucleotide sequence encoding Tey 1, a variant Tey 1, or a fragment of either of the foregoing comprising at least 455 contiguous nucleotides; a vector comprising any of the foregoing, wherein, when the isolated or purifies nucleic acid molecule consists essentially of a nucleotide sequence encoding Tey 1 or a variant thereof, the isolated or purified nucleic acid molecule is optionally part of an encoded fusion protein; a cell comprising and expressing any of the foregoing isolated or purified nucleic acid molecules, optionally in the form of a vector; an isolated or purified polypeptide molecule consisting essentially of an amino acid sequence encoding Tey 1, a variant Tey 1, or at least 6 contiguous amino acids of either of the foregoing; a conjugate or a fusion protein comprising a therapeutically or prophylactically active agent, and an excipient or an adjuvant, a method of treating cancer prophylactically or therapeutically in a mammal comprising administering to the mammal an effective amount of (a) an isolated or purified nucleic acid molecule encoding Tey 1, optionally in the for of a vector, or (b) an isolated or purified Tey 1 polypeptide, optionally in the form of a conjugate or fusion protein, whereupon the mammal is treated for the cancer prophylactically or therapeutically; a method of diagnosing cancer comprising (a) obtaining a test sample from the mammal, and (b) assaying the test sample for the level of Tey 1, wherein a decrease in the level of Tey 1 in the test sample as compared to the level of Tey 1, wherein a decrease in the level of Tey 1 in the test sample as compared to the level of Tey 1 in a control sample is diagnostic for the cancer, and a method of prognosticating cancer in a mammal comprising (a) obtaining a test sample from the mammal, and (b) assaying the test sample for the level of Tey 1, wherein an increase in the level of Tey 1 over time is indicative of a positive prognosis and a decrease in the level of Tey 1 over time is indicative of a negative prognosis.