Process for detecting the existence of mesenchymal chrondrosarcoma

摘要

A process for detecting Mesenchymal Chondrosarcoma in a biological organism, comprising detecting, in a sample that contains Mesenchymal Chondrosarcoma cells obtained from a subject a first product indicative of elevated expression of a fibroblast growth factor receptor gene or a second product indicative of elevated amounts of a fibroblast growth factor receptor (FGFR-L1), wherein detection of said first or second product in elevated expression or amount, respectively, compared to a control sample containing normal or benign Mesenchymal Chondrosarcoma cells indicates the presence of Mesenchymal Chondrosarcoma in said subject. The sample is preferably obtained by a process comprising the steps of: (a) obtaining a tissue sample from a living biological organism, (b) disaggregating said tissue sample to produce disaggregated fragments of tissue sample whose maximum dimension is less than about 5 millimeters, wherein said tissue sample is disaggregated within about 10 minutes of the time said tissue sample is obtained from said biological organism, and (c) disposing said disaggregated tissue fragments in a sterile environment within a container, wherein said sterile environment is comprised of oxygen and a solution comprised of at least one cell type specific viability factor.