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Compositions and methods for modifying gene expression using the promoter of the gene for conjugation to the ubiquitin proteu00ecna soybean plants

机译:使用该基因的启动子与遍在蛋白蛋白大豆植物结合的修饰基因表达的组合物和方法

摘要

Compositions and methods for modifying gene expression using the promoter of the gene for proteu00ecna conjugation to ubiquitin of soybean plants. The present invention includes the description of a new regulatory sequ00fau00eancia fitted to improve the expression of a nucleotide sequ00fau00eancia as structural genes in plants, including monocotil edu00f3neas and dicotyledons.More specifically, the invention relates to sequ00fau00eancias regulatory polinucleotideos isolated from soybean plants that are capable of initiating and activating the transcription of polynucleotides encoding, and the use of these sequ00fau00eancias regulatory modification of transcription of polynucleotides encoding en Du00f3genos and / or heteru00f3logos and production of polypeptides.The invention further describes constructions of DNA that contains the promoter of the gene of the protein conjugation to ubiquitin of soybean plants that is operationally linked to a heterologous gene and / or endogenous. In addition, the invention relates to the use of these buildings in the form of expression vectors, recombinant vectors and in plants, plant cells or protoplastos transgenics.The invention describes a method using such constructs containing the promoter of the gene for conjugation to the ubiquitin protein of soybean plants for the production of plants, plant cells or transgenic protoplastos.
机译:使用该基因的启动子修饰蛋白表达的组合物和方法,该蛋白用于与大豆植物的泛素蛋白偶联。本发明包括对新的调节性seq的描述,其适合于改善核苷酸seq作为植物中的结构基因的表达,所述结构性基因包括monocotil ed和双子叶植物。更具体地,本发明涉及seq从大豆植物中分离出的能够启动和激活编码多核苷酸转录的调控性核苷酸,并利用这些序列对编码en D和/或heter的多核苷酸的转录进行调控修饰。本发明进一步描述了DNA的构建,该DNA包含与大豆植物的遍在蛋白缀合的蛋白质的基因的基因的启动子,其与异源基因和/或内源性连接。另外,本发明涉及这些建筑物以表达载体,重组载体的形式以及在植物,植物细胞或原生质体转基因物中的用途。本发明描述了一种使用含有该基因启动子的这种构建体与泛素结合的方法。大豆植物的蛋白质,用于生产植物,植物细胞或转基因原生质体。

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