Conservative fragment of hemA-asuA actinomycete gene and its use for searching producers of biologically active substances containing CSN unit and isolation of corresponding gene clusters

摘要

the solution provides an isolated dna fragment, which represents the conservative section of hema - asua for aminolevulinu00e1tsyntu00e1zu from streptomyces nodosus ssp. asukaensis.this fragment, vu00fdhodne his part is uu017eitecnou probe for searching the micro organism producing secondary metabolites with c5n unit, in particular manumycinovu00e1 antibiotics.the solution provides a very effective, i.e. a fast and cheap way, search the specific micro organism, in particular the bacterium streptomyces sp., producing secondary metabolites with c5n unit, in particular manumycinovu00e1antibiotika, from a large number of the sample, for example.using hybridization colonies or using automated hybridizacnu00edho or pcr screening.the modified method according to the invention is useful for the search for sequences of gene cluster for biosynthesis metabolite with c5n unit, for example. manumycinovu00fdch antibiotics.the solution also provides a kit for use in molecular biology for zpusobech according to the invention.which contains one or more isolated fragment dna as hybridizacnu00ed probes and / or oligonucleotides as pcr primers.the solution also provides full sequence gene cluster from streptomyces parvulus tu00fc64 biosynthesis manumycinu genome.the biosynthesis gene cluster manumycinu is usable to alert manumycinovu00fdch antibiotics biosynthesis manipulation, both for scientific and industrial cause.