Conservative fragment of hemA-asuA actinomycete gene and its use for searching producers of biologically active substances containing CSN unit and isolation of corresponding gene clusters

摘要

The present invention provides an isolated DNA fragment that is conserved region of the gene Hema-Asua for aminolevulinátsyntázu from Streptomyces nodosus ssp. Asukaensis. This fragment, preferably a portion thereof, is useful probe for searching microorganism producing secondary metabolites C5N unit, in particular manumycinová antibiotics. The present invention thus provides a very effective, i.e. a fast and inexpensive way, searching for specific microorganisms, especially bacteria Streptomyces sp., Producing secondary metabolites C5N unit, in particular manumycinováantibiotika, a large amount of sample, e.g., By using colony hybridization or by using an automated hybridization or PCR screening. Modified according to the invention is useful for searching sequence gene cluster for the biosynthesis of a metabolite having C5N unit, e.g. Manumycinových antibiotics. The present invention also provides a kit for molecular biology, for use in the inventive method, which comprises one or more isolated DNA fragment as hybridization probes and / or oligonucleotides as PCR primers. The present invention also provides a complete sequence of the biosynthetic gene cluster manumycinu from the genome of the Streptomyces parvulus Tü64. Manumycinu biosynthesis gene cluster can be utilized to manipulate manumycinových biosynthesis of antibiotics, both for scientific and industrial purposes.