Isolation, purification and biological characterization of an angiogenesisinhibitor derived from sinica cetorhinus maximum cartilage

摘要

Objective:To study shark cartilage-derived active protein(s) which can inhibit angiogenesis and tumor growth. Methods: Shark cartilage-derived proteins were extracted in 1 mol/L guanidine hydrochloride ; purified Sp8 was obtained by ultra-filtration and Superdex 75 chromatography. Sp8 inhibition on vascular endothelial cell growth, rabbit cornea and chick embryo angiogenesis chorioallantoic membrane (CAM), and growth of transplanted S180 sarcoma in mice were all studied. Results:① inhibited the growth of vascular endothelial cells in vitro, the inhibitory rate was 20% at the concentration of 40 μg/ml. ②Sp8 showed a strong inhibition on angiogenesis, the inhibitory rate reached 72.7% on rabbit cornea and 79% on CAM model. ③Sp8 efficiently reduced the growth of S180 sarcoma in mice, IC50 on this model was less than 100 μg/kg. Conclusion: Sp8 mediates at least part of the anti-tumor activity of shark cartilage through inhibition of tumor-induced angiogenesis.