AN IMPROVED PROCESS FOR THE PREPARATION OF 10-HYDROXY CAMPTOTHECIN USING ASPERGILLUS OCHRACEUS

摘要

An improved process for the preparation of 10-hydroxy camptothecin using aspergillus ochraceus which comprises cultivating novel strain of aspergillus ochraceus having characteristics such as herein described in a conventional nutrient medium additionally containing glucose 2 to 4%, caseine hydrolysate 0.2 to 0.8% corn steep liquor, 0.02 to 0.05% in a known manner, addinq camptothecin in a solvent to the above said qrowinq culture followed by incubating for a period of atleast 24hr, extracting the reaction mixture with a water immiscible organic solvent preferably chloro solvent or esters, removing the solvent to get crude to hydroxy camptothecin and further purifying by known chromatographic methods.