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Engineering of Industrial Aspergillus ochraceus Strains for Improved Steroid 11α-Hydroxylation Efficiency via Overexpression of the 11α-Hydroxylase Gene CYP68J5

机译:通过过量表达11α-羟化酶基因CYP68J5改良工业类As曲菌菌株以提高甾体11α-羟基化效率

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Recombinant strain CYP68J5-TrpC-2 over-expressing the steroid 11α-hydroxylase gene CYP68J5 not only showed a 12% increase in 16,17α-epoxyprogesterone (EP) conversion rates but also shortened the transformation time by 12 h compared with the recipient production strain TCCC41060, indicating that the transcript level of 11α-hydroxylase gene CYP68J5 is a limiting factor for 11α-hydroxylation efficiency of strain TCCC41060 on EP. Thus, increasing steroid 11α-hydroxylase expression is an effective strategy for engineering highly efficient commercial strains for steroid 11α-hydroxylation in A. ochraceus TCCC41060.
机译:过度表达类固醇11α-羟化酶基因CYP68J5的重组菌株CYP68J5-TrpC-2不仅显示16,17α-环氧孕酮(EP)的转化率提高了12%,而且与受体生产菌株相比,转化时间缩短了12 h TCCC41060,表明11α-羟化酶基因CYP68J5的转录水平是限制TCCC41060在EP上的11α-羟化效率的限制因素。因此,增加类固醇11α-羟化酶的表达是工程化effective菜TCTC41060中用于类固醇11α-羟化的高效商业菌株的有效策略。

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