OLIGONUCLEOTIDE PROBE FOR DETECTION AND ENUMERATION OF VIBRIO SPP. IN AQUACULTURE SYSTEMS.

摘要

This invention relates to a genus specific oligonucleotide probe for detection and enumeration of Vibrio spp in aqua-culture systems and shrimp processing industries by colony hybridization technique having sequence based on gene coding for the enzyme DNA gyrase, gyr B having certain unique, sequence that are conserved in members of a genus, wherein the probe has been conjugated with the enzyme alkaline phosphatase (C6) 5' TGTCAGGAAAAAGATCCTGCACTGTC TGA ACT 3' and a protocol for performing colony hybridization using the said oligonucleotide probe.