A Method of Separating all high Molecular Weight Mannose-Binding Lectin (MBL) Molecules

摘要

The invention relates to a process for preparing a high molecular weight lectin composition, in particular comprising the mannose-binding lectin (MBL), suitable for using recombinantly produced lectins as starting material, a high molecular weight lectin composition, a pharmaceutical composition comprising same, as well as the use of the produced composition for the prepa¬ration of a pharmaceutical composition for treating various conditions and diseases. In one aspect there is provided a method for producing a composition comprising a variety of lecting molecules, wherein substantially all of said lecting molecules having a high molecular weight above the molecular weight for dimer lectins, said method comprising, obtaining a lectin preparation comprising lectin molecules having a high molecular weight above the molecular weight for dimer lectins and lectin molecules having a low molecular weight below or equal to the molecular weight for dimer lectins, said preparation having the radio R=Ro, wherein R is the ratio of the concentration of lectin molecules having a high molecular weight above the molecular weight for dimer lectins to the concentration of lectin molecules having a low molecular weight below or equal to the molecular weight for dimer lectins, adding to said preparation a precipitating agent and allowing a precipitate and a supernatant to form, separating said precipitate from said supernatant, obtaining a precipitate fraction having the ratio R=R1 wherein R1Ro, and optionally obtaining a supernatant fraction having the ratio R=R2, wherein R2Ro, and obtaining a composition comprising the lectin molecules of the precipitate fraction.