A simple plant high-molecular-weight DNA extraction method suitable for single-molecule technologies

摘要

The high-molecular-weight genomic DNA fractionation by Pulsed-field gel system (CHEF system; initial SW time: 1.0, final SW time: 40.0; voltage, 6 V/cm; included angle: 120; running at 14 °C for 16–24 h in 0.5 × TBE buffer). Lane 1, 5, 8 and 11 Lamda PFG Marker; lane 2, cotton CSX8303; lane 3, blackgrass grass; Lane 4, cotton UGA230; lane 6 and 7, strawberry genotype Royal Royce; lane 9, cotton Coker, lane 10, cotton 94-L25 genomic DNA; lane 12, High concentration (900 ng/µl) cotton Jin668 gDNA stored at − 20 °C for 16 month, lane 13, Low concentration (200 ng/µl) cotton Jin668 gDNA stored at − 20 °C for 16 month