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A METHOD FOR ISOLATING AT LEAST ONE MANNOSE-BINDING LECTIN (MBL)
A METHOD FOR ISOLATING AT LEAST ONE MANNOSE-BINDING LECTIN (MBL)
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机译:至少一个绑定甘露聚糖的蛋白(MBL)的分离方法
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摘要
"A method for isolating at least one Mannose-binding lectin (MBL)" The present invention relates to a method for isolating at least one Mannose-binding lection (MBL) from a preparation comprising a variety of lection molecules, comprising -establishing a solid support being derivatized with at least one amino sugar having a primary amino group positioned as a reactive part in one part of the molecule and an MBL binding site in another part of the molecule, and wherein said amino sugar is coupled through the amino group to the solid support, -applying the preparation to said support, -allowing the lectins to bind to the amino sugar coupled to the solid support, -washing the solid support with a waqshing liquid removing unbound contaminants, and -optionally recovering said lectin(s) from the solid support.
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