ANNEALING CONTROL PRIMER AND METHODS INVOLVING NUCLEIC ACID AMPLIFICATION WITH THE ANNEALING CONTROL PRIMER

摘要

An annealing control primer for improving annealing specificity in nucleic acid amplification, which comprises: (a) a 3' -end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5' -end portion having a pre-selected arbitary nucleotide sequence; and (c) a regulator portion positioned between said 3' -end portion and said 5' -end portion comprising at least two universal bases or non-discriminatory base analogs, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperaturer.