chimeric influenza virus, recombinant DNA molecule, method for producing an immunogenic formulation, chimeric ndv, and method for inducing an immune response to two infectious agents

摘要

CHEMICAL INFLUENCE VIRUS, RECOMBINANT DNA MOLLECLE, METHOD TO PRODUCE IMMUNOGENAL FORMULATION, CHEMICAL NDV, AND METHOD TO INDUCES AN IMMUNE RESPONSE TO TWO INFECTIOUS AGENTS. The present invention provides chimeric negative strand RNA viruses that enable an individual, for example a bird, to be immunized against two infectious agents by use of a single chimeric virus of the invention. Particularly, the present invention provides chimeric influenza viruses engineered to express and incorporate into their virions a fusion protein comprising an ectodomain of an infectious agent protein and the transmembrane and cytoplasmic domain of an influenza virus protein. These chimeric viruses induce an immune response against influenza viruses and the infectious agent. The present invention also provides Newcastle disease chimeric virus (NDV) engineered to express and incorporate into its virions a fusion protein comprising the ectodomain of an infectious agent protein and the transmembrane and cytoplasmic domain of an NDV protein. These chimeric viruses induce an immune response against NDV and the infectious agent.