Nucleic acid probes and broad-range primers of regions in topoisomerase genes, and methods in which they are used.

摘要

A diagnostic method for detecting and identifying bacterial species causing infections from a clinical sample, characterized in that a) amplifying a DNA isolated from said clinical sample using a mixture of DNA primers that comprises sequences that hybridize with the sequences that originate from conserved regions of genes encoding topoisomerases that, especially gyrB / parE, of bacterial species causing said infections, said sequences comprising SEQ ID NOs 76 and 77 or with complementary sequences thereof. b) contacts the amplified DNA with a desired combination of oligonucleotide probe sequences that hybridize under normal hybridization with hypervariable regions situated near said conserved regions of genes encoding topoisomerases, especially gyrB / parE, of bacterial species that cause these infections, said specific sequences being bacterial species in these hybridization conditions, and c) detecting the formation of a possible hybridization complex.