SEPARATION AND CHARACTERIZATION PROCESS OF POTENTIALLY PRESENT FUNCTIONS IN A BIOLOGICAL SAMPLE CONTAINING NUCLEIC ACIDS.

摘要

Screening process of a biological sample containing nucleic acids, characterized in that it comprises the following steps: a) the determination of the function to be screened, b) the preparation of nucleic acid fragments from said sample, c) the association of each one of said fragments with a vector molecule, d) the isolation of each fragment associated with a vector molecule or with a part of each construction consisting of a fragment associated with a vector molecule, from step (c) e) the treatment in a acellular system of each fragment associated with a vector molecule or with a part of each construct consisting of a fragment associated with a vector molecule of step (d) to obtain transcripts, f) proof of the function determined in step (a) of the transcripts obtained in step (e) or of the proteins for which they encode after the translation of said transcripts.