Lysyl-tRNA compositions and orthogonal aminoacyl-tRNA synthetase pairs and uses thereof.

摘要

Translation system comprising: a lysyl orthogonal tRNA (lysyl O-tRNA) or modified variant thereof and a tRNA aminoacyl synthetase orthogonal (O-RS) that loads preferentially the lysyl O-tRNA or modified variant thereof with homoglutamine; wherein the O-RS and lysyl O-tRNA or modified variant thereof have an efficiency of at least 50% to suppress a selector codon or frameshift reading as PhDeltaAD having the sequence of amino acids of SEQ ID NO: 28, or an I41 and / or S268 of PhDeltaAD, in combination with an O-tRNA of SEQ ID NO: 26.