Extraction, amplification and sequential hybridization of fungal DNA and methods for detecting fungal cells in clinical material.

摘要

FOR FUNGAL CELLS IN SCREENING CLINIC MATERIAL fungal DNA is extracted TOTAL BLOOD AND EXTRACTED fungal DNA tested BELOW. THANKS TO THIS TEST CAN DETERMINE THE EXISTENCE OF A fungal infection. DIAGNOSTICS FOR OTHER SPECIES ARE DETERMINED FROM FUNGAL fungal DNA extracted. PROCEDURE FOR REMOVING THE fungal DNA FROM WHOLE BLOOD includes isolating fungal cells essentially intact BLOOD AND EXTRACTION OF TOTAL DNA from isolated cells. For detection of fungal DNA it is amplified at least one segment of DNA fungal DETERMINE optionally detecting amplification products. IDENTIFYING to continue the fungal species, SECTIONS ARE DETERMINED Nucleotide sequences CHARACTERISTICS OF fungal species.