首页>
外国专利>
Antigen capture anti-dengue IgA ELISA (ACA-ELISA) for the detection of a flavivirus specific antibody
Antigen capture anti-dengue IgA ELISA (ACA-ELISA) for the detection of a flavivirus specific antibody
展开▼
机译:抗原捕获抗登革热IgA ELISA(ACA-ELISA)用于检测黄病毒特异性抗体
展开▼
页面导航
摘要
著录项
相似文献
摘要
An antigen capture IgA Enzyme Linked Immunosorbent Assay (ACA-ELISA) was developed for the detection of anti-flavivirus IgA. The assay utilizes flavivirus lysate antigen, preferably dengue virus lysate antigen captured by a monoclonal antibody. Captured anti-flavivirus IgA from test sera are preferably detected using rabbit anti-IgA conjugated with a reporter group such as horseradish peroxidase (HRP). The assay was found to be at least 8 times more sensitive than anti-human IgA capture ELISA (AAC-ELISA). The ACA-ELISA, based either on serum or saliva, was found to be more sensitive and rapid compared to the "gold standard" anti-dengue IgM detection technique and can be utilized as a diagnostic tool for the confirmation of dengue in the early phase of infection.
展开▼