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METHOD FOR DETERMINATION OF CELL VIABILITY BY USING FLOW CYTOMETRY WITH FIXED VOLUME ACQUISITION
METHOD FOR DETERMINATION OF CELL VIABILITY BY USING FLOW CYTOMETRY WITH FIXED VOLUME ACQUISITION
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机译:固定体积获取的流式细胞仪测定细胞活力的方法
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摘要
The present invention provides a method for comparing the content of a specific cell subpopulation in cell suspension samples by determining the count of cells of said subpopulation per unit volume in each sample, using a flow cytometer with fixed volume acquisition, but without the use of an internal or external standard. Thus, the effect of a test compound on cell viability can be determined, especially the cytotoxic or cytostatic effect of anticancer drugs. The method is a useful tool for predicting the biological activity of cytotoxic or cytostatic compounds on cells of the same kind as the cells in the sample. Furthermore, a kit for performing the method is provided.
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