Method for determination of cell viability by using flow cytometry with fixed volume acquisition

摘要

Present invention provides a method for comparing the content of a specific cell subpopulation in cell suspension samples by determining the count of cells of said subpopulation per unit volume in each sample, using a flow cytometer with fixed volume acquisition, but without the use of an internal or external standard. Thus, the effect of a test compound on cell viability can be determined, especially the cytotoxic or cytostatic effect of anticancer drugs. The method is a useful tool for predicting the biological activity of cytotoxic or cytostatic compounds on cells of the same kind as the cells in the sample. Furthermore, a kit for performing the method is provided.