Using Flow Cytometry to Analyze Cell Viability of E. coli in Water during Controlled Release Chemical Oxidation Remediation.

摘要

Finding alternative ways to treat water to conserve energy, time and money while protecting the environment is imperative due to a depleting freshwater supply. However, many issues surround the reuse of greywater, regardless of the source; the presence of pathogens in the greywater presents an issue that will need to be treated prior to reuse. This research focuses on using a Controlled Release Biodegradable Polymer (CRBP) containing oxidants, KMnO4 and Oxone, to treat simulated greywater while studying the viability of E. coli within the water sample by using flow cytometry analysis. It was determined that the changing release rate of the Oxone CRBP corresponded with the viability of the E. coli cells in the contaminated water samples.;Recommended treatment times for each type of oxidant infused polymer was determined as follows: (1) Initial use of Oxone infused CRBP should have a minimum of 1.26 days (approximately 30 hours, 15 minutes) release time with E. coli contaminated water. (2) Use after the initial utilization of the Oxone CRBP should have a minimum release time of 2.54 days (approximately 61 hours) to properly treat E. coli contaminated water. (3) Initial use of KMnO4 CRBP should have a minimum of 1.18 days (approximately 28 hours, 24 minutes) of contact time with contaminated water. (4) After the initial use of the KMnO4 CRBP, the pellets should be allowed to release for a minimum period of 4.18 days (approximately 100.5 hours) to adequately disinfect E. coli contaminated water.