METHOD FOR ENUMERATING MAMMALIAN CELL MICRONUCLEI WITH AN EMPHASIS ON DIFFERENTIALLY STAINING MICRONUCLEI AND THE CHROMATIN OF DEAD AND DYING CELLS

摘要

The present invention relates to a kind of for enumerating the micronucleus of mammalian cell, while distinguishing micronucleus from above-mentioned chromatin death and dye cell. This method is distinguished from dead and dye cell using setting off coloration and dyeing matter, chromatin from micronucleus and core, therefore can fluorescent emission based on detection and scatterer in the light source for being exposed to exciting light. It counts micronucleus event and can be used for assessing the potential chemical agents of DNA-damaging, the influence of the potential physical agents of DNA-damaging relative to nucleus number mesh, reagent can change the effect of the DNA damage of endogenous induction, act on behalf of the impaired of the DNA induced with capable of changing external source. Kit for carrying out the present invention is also disclosed.