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METHOD FOR ENUMERATING MAMMALIAN CELL MICRONUCLEI WITH AN EMPHASIS ON DIFFERENTIALLY STAINING MICRONUCLEI AND THE CHROMATIN OF DEAD AND DYING CELLS
METHOD FOR ENUMERATING MAMMALIAN CELL MICRONUCLEI WITH AN EMPHASIS ON DIFFERENTIALLY STAINING MICRONUCLEI AND THE CHROMATIN OF DEAD AND DYING CELLS
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机译:用染色剂对死细胞和染色质分别染色微染色的哺乳动物细胞微核的方法
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摘要
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.
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