BIOLOGICAL MICROCHIP FOR MULTIPLE PARALLEL IMMUNOASSAY OF COMPOUNDS AND IMMUNOASSAY METHODS USING SAID MICROCHIP

摘要

The present invention relates to biochemistry, medicine, and molecular biology, in particular to analytical biochemistry and immunochemical assay, and is concerned with a biological microchip for multiple parallel detection and quantitative determination of different compounds. The biochip comprises an array of three-dimensional hydrogel elements which have a predetermined volume, formed on a support by the method of photo- or chemically induced polymerization, and containing biological molecules of the same or different nature (ligands). The invention provides a method for detecting compounds on the biological microchip, comprising identification thereof by mass spectrometry techniques directly on the hydrogel element of the microchip and a method for detecting immunoassay results, consisting in carrying an immuno-PCR and registering the results thereof on the same microchip. The invention also relates to a method of multiple parallel immunoassay of compounds on a biological microchip. Biological microchips for immunoassay and a method of carrying out multiple parallel analysis of compounds can find application in analyzing a wide range of high-molecular and low-molecular compounds, in medicine, pharmacology, food industry, environmental protection, in research work, particularly in proteomics.