首页> 外国专利> Transgenic sweetpotato plants with shade tolerance and process for producing the same

Transgenic sweetpotato plants with shade tolerance and process for producing the same

机译:具有耐荫性的转基因甘薯植物及其生产方法

摘要

A transgenic sweet potato is provided to utilize light efficiently by decreasing or inhibiting competition in a plant or between plants due to short height and internode, increased number of leave, thick stem, and dark green leaves, promote the strong growth of the plant, and increase the resistance against biological and non-biological stress, thereby increasing the productivity of the sweet potato. A transgenic sweet potato(Ipomoea batatas) is redifferentiated from an embryogenic callus of a sweet potato transformed by a recombinant vector including a phytochrome A gene of mutated Avena sativa in which a number 598 amino acid serine codon(AGT) is substituted with an alanine codon(GCT) and the expression of the gene is under the control of a CaMV35S promoter or a microorganism transformed by the recombinant vector; and has the inhibited shade avoidance or the increased shade tolerance. A method for preparing the transgenic sweet potato comprises the steps of: (a) constructing the recombinant vector; (b) after transforming the embryogenic callus of the sweet potato with the recombinant vector through particle bombardment or Agrobacterium tumefaciens technique, selecting the transformed callus; and (c) after redifferentiating the transgenic potato having the inhibited shade avoidance or the increased shade tolerance from the transformed callus, selecting the transgenic sweet potato.
机译:提供了一种转基因甘薯,可通过减少或抑制植物高度或节间高度,叶片数目增加,茎粗和深绿色的叶片而减少或抑制植物之间或植物之间的竞争来有效利用光,促进植物的强壮生长,以及增加对生物和非生物胁迫的抗性,从而提高甘薯的生产力。从甘薯的胚性愈伤组织中再分化出一个转基因甘薯(Ipomoea batatas),该重组载体由包含突变的Avena sativa的植物色素A基因的重组载体转化而成,其中598个氨基酸的丝氨酸密码子(AGT)被丙氨酸密码子取代。 (GCT),并且该基因的表达在CaMV35S启动子或重组载体转化的微生物的控制下;并具有避免遮阴或增加遮阴公差的能力。一种制备转基因甘薯的方法,包括以下步骤:(a)构建重组载体; (b)用重组载体通过粒子轰击或根癌农杆菌技术转化甘薯的胚性愈伤组织后,选择转化的愈伤组织; (c)从转化的愈伤组织中重新分化出具有避光抑制作用或增强的耐荫性的转基因马铃薯,然后选择转基因甘薯。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号