RECOMBINANT PROTEIN OF ENZOOTIC BOVINE LEUKEMIA VIRUS EXPRESSED IN A INSECT CELL AND THE DIAGNOSTIC METHOD

摘要

Recombinant proteins of enzootic bovine leukemia virus are provided to improve specificity and sensitivity of detection as compared with conventional antigens by expressing the envelope glycoproteins in baculovirus and secreting the envelope glycoproteins to insect cells. A method for producing a recombinant protein of enzootic bovine leukemia virus expressed in an insect cell comprises the steps of: (1) cloning an envelop glycoprotein of enzootic bovine leukemia virus from the blood of enzootic bovine leukemia positive cows to baculovirus and sequencing the cloned protein; (2) transforming an insect cell with a recombinant virus gene of SEQ ID NO:1 to express a recombinant protein of SEQ ID NO:2; and (3) purifying and concentrating the recombinant protein produced from the insect cell, wherein the recombinant virus gene of SEQ ID NO:1 is prepared by recombination of envelop glycoprotein genes including a whole gp51 gene and a part of gp30 gene.