CENTRIFUGAL FORCE-BASED MICROFLUIDIC DEVICE FOR NUCLEIC ACID DETECTION AND MICROFLUIDIC SYSTEM COMPRISING THE DEVICE

摘要

A centrifugal force-based microfluidic device for detecting nucleic acids is provided to perform automatically a series of operations including isolation, purification and concentration of a target cell from a biological sample, extraction of nucleic acids from the concentrated target cell and detection of genetic properties by PCR(polymerase chain reaction) in one device rapidly. A centrifugal force-based microfluidic device(101) for detecting nucleic acids comprises: a rotatable platform(100); a target cell nucleic acid extraction portion which, in a microfluidic structure located within the platform, mixes microparticles capturing a target cell in the surface with a biological sample, isolates and purifies the microparticles capturing the target cells, and destroys the target cells by using the electromagnetic wave irradiated to the microparticles from the outside; and a PCR(polymerase chain reaction) unit(90) which, in the microfluidic structure located within the platform and connected to the target cell nucleic acid extraction portion, mixes the nucleic acid solution with PCR solution, and performs PCR by the thermal cycling through heat exchange between the mixture and a temperature control unit located in the outside of the platform, wherein the PCR unit contains a reaction chamber(80) for storing the PCR solution and mixes with the target cell nucleic acid solution from the target cell nucleic acid extraction portion, and a PCR chamber(92) for receiving the mixture of the reaction solution and nucleic acid solution by connecting to the reaction solution chamber. Further, the PCR chamber is sealed up while polymerase chain-reacts.