PRODUCTION OF TRANSGENIC CLONED EMBRYO BY SOMATIC CELL NUCLEUS TRANSFECTED WITH HGM-CSF GENE AND PREPARATION METHOD OF THE SAME

摘要

A production method of a transgenic cloned embryo having a goat somatic cell nucleus transfected with hGM-CSF(human granulocyte macrophage-colony stimulating factor) gene is provided to produce a cloned goat expressing the hGM-CSF from the mammary gland, thereby improving the production yield of hGM-CSF. A production method of a transgenic cloned goat embryo comprises the steps of: (1) transforming a somatic cell of goat with a recombinant expression vector containing an hGM-CSF gene to prepare a nucleus donor cell; (2) collecting an ovum from the goat to prepare a nucleus recipient ovum; and (3) denucleating the recipient ovum, transplanting the nucleus donor cell into the recipient ovum and fusing them to prepare the transgenic cloned embryo.