PCR PRIMER FOR SIRE SELECTION USING A-FABP GENOTYPE ASSOCIATED WITH INTRAMUSCULAR FAT AND GROWTH TRAITS IN PIGS AND THE SELECTION METHOD USING THEM

摘要

A PCR(polymerase chain reaction) primer for selection of breeding pigs is provided to select a breeding pig having A-FABP(adipocyte fatty acid binding protein) genotype capable of improving intramuscular fat in fed pigs and growth traits in Duroc breed, so that fed pigs having high intramuscular fat and rapid growth traits are produced. An FBp primer set for selection of breeding pigs consists of an FBp forward primer having the nucleotide sequence of 5'-CTGTTCCCCAAAGCCTCTGC-3' and an FBp reverse primer having the nucleotide sequence of 5'-AGCTTAGAAGCCGTAACCTT-3'. A method for selection of breeding pigs comprises the steps of: performing PCR of DNA extracted from the blood or hair root of pigs with the FBp primer set; storing the PCR products at 4 deg. C; digesting the PCR products with a restriction enzyme HinfI and leaving them in an incubator at 37 deg. C for 4 hours or more; and subjecting the digested reaction solution to 10-15% polyacrylamide gel electrophoresis and staining the developed gel with ethidium bromide solution to determine the genotype.