METHOD FOR PRODUCTION OF BACILLUS SUBTILIS SUBSP. SUBTILIS A-53 WITH RICE HULL

摘要

Method hay bacillus subsp. for productionWithered grass one -53 is arranged to improve with rice husk increases hay bacillus subsp.The productivity of withered grass one -53 and cellulase, reduces environmental pollution, and enhances economic effect compared with solid culture with rice husk and uses Liquid Culture. One hay bacillus subsp.Withered grass one -53 (KAC 91179P) is cultured in a broth bouillon, contains a carbon source, is selected from rice husk, rice bran, maltose, fructose, sugar and glucose, one nitrogen source, selected from yeast extract, peptone, malt extract, rouge peptone, ammonium sulfate and ammonium nitrate, inorganic salts, including 0.5% (w/w) K2HPO4,0.1% (w/w) NaCl, 0.02% (w/w) MgSO4.7H2O and 0.06% (w/w) (NH4) 2SO4 is 3-5 days at 30-40 DEG C. In addition, carbon source includes 1 to 10% (w/w) of husk, nitrogen source is the 0. of yeast extract05 to 1% (w/w).