STREPTOMYCES SP. STRAIN HAVING AN ALGINATE HYDROLYSIS ACTIVITY AND ALGINATE LYASE

摘要

A Streptomyces sp. strain with the alginate hydrolysis activity is provided to grow well at a temperature of 20-30 deg.C, which is lower than an optimum growth temperature of land microorganisms, thereby being used for hydrolyzing the alginate with no special process. An alginate lyase derived from the same strain is provided to show high activity in a weak acidic condition and excellent hydrolyzing activity on polyguluronic acid block of alginate(pG block), polymanuronic acid block of the alginate(pM block), polyguluronic acid-polymanuronic acid block of the alginate(poly M-G block / poly G-M block) and sodium alginate, thereby being useful for producing alginate oligomer with no additive process for pH adjustment. A Streptomyces sp. strain having the alginate hydrolyzing activity is deposited as a deposition no. KCTC 11091BP. An alginate lyase consists of an amino acid sequence described as SEQ ID : NO. 5. A polynucleotide encoding the alginate lyase consists of a DNA sequence encoding the amino acid sequence described as SEQ ID : NO. 5. A transformant, which is Escherichia coli, bacillus sp., Pseudomonas sp. strain or yeast, is transformed by a vector including the polynucleotide encoding the alginate lyase.