Antioxidant activity and free radical scavenging activities of Streptomyces sp. strain MJM 10778

摘要

Objective:To investigate the antioxidant activity of soil-borne actinobacteria. Methods:The total phenolic contents, the level of antioxidant potential byDPPH radical scavenging activity,NO scavenging activity, andABTS radical scavenging activity in ethyl acetate extract were determined.Results:The16S rDNA sequencing analysis revealed thatStreptomyces sp. strainMJM10778, which was isolated fromHambakMountain,Korea, has99.9% similarity to Streptomyces misionensis(S. misionensis)NBRC13063.The physiological and the morphological test revealed that the strainMJM10778 has different characteristics from the strainNBRC13063. The entire antioxidant assay with the ethyl acetate extract displayed good radical scavenging activity.TheIC50 values of the strainMJM10778 extract onDPPH,NO, andABTS radicals were identified to be92.8 μg/mL,0.02 μg/mL, and134.9 μg/mL, respectively.The ethyl acetate extract of the strainMJM10778 showed an81.50% of cell viability at100 μg/mL inRaw264.7 cell viability assay.Conclusions:The results obtained suggest that the ethyl acetate extract ofStreptomyces sp. strainMJM10778 could be considered as a potential source of drug for the diseases that is caused by free radicals with its anti-oxidant activities and low cytotoxicity.