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METHODS FOR PREPARING TRUNCATED PROTEINS FROM NMD-ESCAPE NUCLEIC ACID MOLECULES
METHODS FOR PREPARING TRUNCATED PROTEINS FROM NMD-ESCAPE NUCLEIC ACID MOLECULES
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机译:由NMD-蔗糖核酸分子制备截短蛋白的方法
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摘要
A method for preparing a truncated protein from an NMD(nonsense-mediated mRNA decay)-escape nucleic acid molecule is provided to obtain the truncated protein, which is used as a tumor antigen and used for developing a dendritic cell therapeutic agent. A method for screening a compound inhibiting a nonsense-mediated translational repression(NMTR) is provided to be used in order to screen a material capable of generating the truncated protein from the NMD-escape nucleic acid molecule through a high-through screening. A method for preparing a truncated protein from an NMD-escape nucleic acid molecule comprises a step of inhibiting an NMTR in a cell by preventing an mRNA of the NMD-escape nucleic acid molecule from being coupled to a polysome, wherein the NMD unable to express the truncated protein is BCL2-associated X protein(BAX), human mutS homologue 3(hMSH3), TATA box binding protein(TBP)-associated factor 1B(TAF1B) or transforming growth factor beta receptor 2(TGFBR2). A method for screening a compound inhibiting the NMTR comprises the steps of: (a) transfecting an NMD-escape nucleic acid molecule having a premature termination codon into a cell; (b) contacting the cell with a candidate compound; and (c) detecting expression of the truncated protein from the nucleic acid molecule.
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