High Throughput Screening Method of Binding Inhibitor between Caspase3 and xAIP and Binding Inhibitor Screened Thereby

摘要

The present invention relates to an ultrafast screening method of caspase3 and xIAP binding inhibitors and to chromomycin A3 screened by the method, and more specifically, to detecting an interaction between caspase3 and xIAP. Reacting a caspase3 or xIAP with a candidate substance that inhibits the binding between caspase3 and xIAP on a biochip, and selecting a candidate substance that inhibits the binding between caspase3 and xIAP as an anticancer substance; and It relates to an inhibitor of binding between caspase3 and xIAP screened by the above method.;According to the present invention, it is possible to develop a target-oriented anticancer agent targeting the apoptosis-related proteins caspase3 and xIAP, which can be applied to customized medicines and concurrent therapies, and using the present invention, screened glycoside antibiotics Chromomycin may be used as a therapeutic agent for hematologic and solid cancers because of its inhibitory activity against the apoptosis-related proteins caspase3 and xIAP.;Apoptosis, glycoside antibiotics, ultrafast screening methods